Long-lasting glow in the excitation light irradiation

Biological applications 

Long-lasting glow in the excitation light irradiation stopped emitting substance can still ongoing phenomenon. Long afterglow luminescent material does not contain toxic heavy metals, can inspire before detection and imaging, glow in the dark dye‍ biological sensing and imaging to achieve in the "free excitation" condition, thus effectively avoiding the interference generated in situ inspired background. Despite the long afterglow luminescent materials have the advantage, but until 2007 only the afterglow materials used in biological sensing and imaging reports. Chermont sol-gel method, such as the use of high-temperature synthesis of silicates with near-infrared fluorescent afterglow nanomaterials and applied vivo imaging (Figure 3). The method was successfully avoids potential damage to organisms traditional fluorescence excitation light source analysis methods, the first time in vivo "free excited" fluorescence imaging. 

Ca0.2Zn0.9Mg0.9Si2O6: Eu, Dy, Mn afterglow nanoparticles in vivo imaging results (A) prior to injection, the first nanoparticle 6 W UV lamp placed 2 cm at the excitation 5 min. (B) respectively, followed by three backs of mice injected with different concentrations in different positions (100, 10 and 1μg/mL) nano-particle suspension (20μl). For 2 min using the acquisition time, wherein fluorescence is easily glow in the dark material‍ monitored two larger doses (2μg and 200 ng) issued. Nano-particles (C) of the mice after intramuscular injection and dispersion of 90 s 

Li Zhan-jun and his collaborators reported the use of MCM-41 as a template for preparing long afterglow materials for biological and cell imaging (Figure 6), and its clever use of MCM as a template, the use of MCM-41 can get clever morphology better spherical nanoparticles, although the material during sintering temperature longer prone to collapse, but the resulting sintered material size and particle size on the nm scale dispersion better. 

? PEG-coated nanoparticles for in vivo afterglow imaging 

2012 Maldiney et PLNP of PEG-coated material and the surface-modified with biotin, and between the avidin biotin interaction so strong that the dissociation constant Kw 10 or less. They Task Force to detect the use of this material is highly expressed avidin surface of glioma cells, the effect is very sensitive and can be used glioma cell imaging (Figure 7).

Figure 7 biotin and PEG-coated nanoparticles for glow in the dark stones‍ afterglow avidin detection sensor detection and BT4C cells